Journal: Journal of Cellular and Molecular Medicine

Article Title: CircFOXP1/FOXP1 promotes osteogenic differentiation in adipose‐derived mesenchymal stem cells and bone regeneration in osteoporosis via miR‐33a‐5p

doi: 10.1111/jcmm.15792

Figure Lengend Snippet: CircRNA expression profiles in OP patients. A, Cluster heat map for top 40 differentially expressed circRNAs (20 up‐regulated and 20 down‐regulated) in OP and non‐OP bone tissues. Red colour represents high expression level, green colour represents low expression level. B and C, Relative expression for six most up‐regulated (B) and six most down‐regulated (C) circRNAs validated by qRT‐PCR. D, Relative expression of circFOXP1 in OP (n = 20) and non‐OP (n = 20) bone tissues determined by qRT‐PCR. E, FOXP1 mRNA expression level in OP (n = 20) and non‐OP (n = 20) bone tissues. F, CircFOXP1 is positively correlated with FOXP1 mRNA in OP tissues. G and H, FOXP1 mRNA (G) and protein (H) levels determined by qRT‐PCR and Western blotting in hASCs with circFOXP1 overexpression or knockdown. All experiments were performed at least three times. Untreated hASCs were used as negative control (NC). ** P < 0.01 vs NC group. OP, osteoporosis; hASCs, human adipose‐derived stem cells

Article Snippet: Labelled cRNAs were hybridized onto the Arraystar Human circRNA Array V1.0.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Over Expression, Knockdown, Negative Control, Derivative Assay

Journal: American Journal of Translational Research

Article Title: Circ_0039569 promotes renal cell carcinoma growth and metastasis by regulating miR-34a-5p/CCL22

doi:

Figure Lengend Snippet: CircRNA expression profile in RCC tissues. A. Heat map showed the significantly expressed circRNAs with 5 folds were presented. In the heat map, red color: the upregulated circRNAs with high fold changes; green color: the downregulated circRNAs with high fold changes. B. Scatter plots are used to evaluate the difference in the expression of circRNAs between experiment group and control group. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). The middle green line refers to no difference between the two groups. And the flank green lines represent 2.0 fold changes. The circRNAs above the top green line and below the bottom green line indicate more than 2.0 fold changes between two groups. C. Six circRNAs including circ_0036840, circ_0082616, circ_0017724, circ_0039569, circ_0039569, and circ_0062705 were chosen for validation in the RCC tissues using qRT-PCR. D. Six circRNAs including circ_0086458, circ_0019692, circ_0037923, circ_0040865, circ_0051398, circ_0039151 were chosen for validation in the RCC tissues using qRT-PCR. Data were expressed as mean ± SD. **P < represents statistical difference.

Article Snippet: Finally, these labeled RNAs were hybridized using Arraystar mouse circRNA Array (V1.0, Arraystar), and scanned by the Agilent Scanner G2505C.

Techniques: Expressing, Quantitative RT-PCR